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ehei  (New England Biolabs)


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  • 94

    Structured Review

    New England Biolabs ehei
    Ehei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ehei/SfoI/pmc08963876-156-33-34
    Average 94 stars, based on 62 article reviews
    ehei - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: MicroRNA-138 controls hippocampal interneuron function and short-term memory in mice
    Article Snippet: Dlx-138 sponge and Dlx-control sponge were cloned into a modified mCaMKIIα-mCherry-WPRE-hGHp(A) (p199, Viral vector facility of the Neuroscience Center Zurich) backbone where 138-sponge and 138-sponge reversed (control-sponge) were previously inserted using MiR-138 sponge vector as template. .. The backbone was opened with MluI (NEB: R0198S) and KpnI (NEB: R0142). pAAV-mDlx-GFP-Fishell-1 (gift from Gordon Fishell; Addgene plasmid #83900) was used to cut out Dlx enhancer and HBB promoter with MluI and EheI (NEB: R0606S). ..

    Article Title: Urokinase Stimulates Human Vascular Smooth Muscle Cell Migration via a Phosphatidylinositol 3-Kinase-Tyk2 Interaction
    Article Snippet: Wortmannin and LY294002 were from Sigma; uPA and ATF were from Loxo (Dossenheim, Germany). .. Restriction endonucleases HindIII, XbaI, PacI, EheI, and T4 DNA ligase were from New England BioLabs (Beverly, CA); the Expand High Fidelity PCR system was from Roche Diagnostics GmbH (Mannheim, Germany); plasmid pCRI2.1TOPO was purchased from Invitrogen (Groningen, The Netherlands); and plasmids pTGBKCMV and pAD1 were from HepaVec (Berlin, Germany). .. Antibodies—Mono- and polyclonal anti-phosphotyrosine antibodies were from Affinity Research Products Ltd. (Exeter, UK) and Pierce; mono- and polyclonal anti-p85 PI3-K antibodies were from Transduction Laboratories (Lexington, KY) and Upstate Biotechnology, Inc. Mono- and polyclonal anti-Jak-kinase antibodies were purchased from Transduction Laboratories and Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

    Polymerase Chain Reaction:

    Article Title: Urokinase Stimulates Human Vascular Smooth Muscle Cell Migration via a Phosphatidylinositol 3-Kinase-Tyk2 Interaction
    Article Snippet: Wortmannin and LY294002 were from Sigma; uPA and ATF were from Loxo (Dossenheim, Germany). .. Restriction endonucleases HindIII, XbaI, PacI, EheI, and T4 DNA ligase were from New England BioLabs (Beverly, CA); the Expand High Fidelity PCR system was from Roche Diagnostics GmbH (Mannheim, Germany); plasmid pCRI2.1TOPO was purchased from Invitrogen (Groningen, The Netherlands); and plasmids pTGBKCMV and pAD1 were from HepaVec (Berlin, Germany). .. Antibodies—Mono- and polyclonal anti-phosphotyrosine antibodies were from Affinity Research Products Ltd. (Exeter, UK) and Pierce; mono- and polyclonal anti-p85 PI3-K antibodies were from Transduction Laboratories (Lexington, KY) and Upstate Biotechnology, Inc. Mono- and polyclonal anti-Jak-kinase antibodies were purchased from Transduction Laboratories and Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).



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    Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.
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    Image Search Results


    Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Journal: International Journal of Molecular Sciences

    Article Title: Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

    doi: 10.3390/ijms21155196

    Figure Lengend Snippet: Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Article Snippet: S144 DNA was digested with the FastDigest restriction enzymes EheI, MauBI, PacI, Cfr42I, XhoI, NotI, Bsp120I, SspI and SspDI (respectively, FD0443, FD2084, FD2204, ER0201, FD0694, FD0596, FD0134, FD0774, FD0774 and ER2191 from Thermo Fisher).

    Techniques: Methylation